Coding

Part:BBa_K4011000:Design

Designed by: Zixiang Zhou   Group: iGEM21_LINKS_China   (2021-10-21)


CBM3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 91
    Illegal AgeI site found at 301
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. All codons were optimized for S. cerevisiae or E. coli based on S. cerevisiae or E. coli codon bias

2. Flexible linker GSGGGS used between first CBM3 and NT2RepCT, flexible linker GGGGS used between NT2RepCT and second CBM3.



Source

CBM3 is derived from Ruminiclostridium thermocellum.

References